Matchmaker Insert Check PCR Mix 2 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, dNTPs and buffer; simply add cells to the mix and perform 30 cycles of PCR. Matchmaker Insert Check PCR Mix 2 is designed to be used with our Matchmaker Gold Yeast One- and Two-Hybrid Screening Systems (Cat. Nos. 630491 and 630489, respectively) to amplify and screen cDNA inserts from prey/library plasmids following yeast one- and two-hybrid screening. The Mix is compatible with GAL4 AD libraries constructed in the following vectors: pGADT7, pGADT7-Rec, pGADT7- Rec2, pGAD424, pACT, pACT2, pGAD GH, pGAD GL, and pGAD10.
Overview
- Quick, easy PCR amplification directly from yeast colonies
- Complete premix contains primers, dNTPs, and buffer—just add your cells and go!
- Primers are also available separately
Applications
- Yeast two-hybrid screening
- Yeast one-hybrid screening
