PCR primers for amplifying and sequencing cDNA inserts in GAL4 Activation Domain (AD) Vectors by either long-distance PCR (LD PCR) or conventional PCR (when the expected insert size is less than 2–3 kb). This Amplimer Set can be used with libraries in the following GAL4 AD Vectors: pGAD10, pGAD424, pGAD GL, pGAD GH, pACT, λACT, pACT2, and λACT2.
Overview
- Quick, easy PCR amplification directly from yeast colonies
- Complete premix contains primers, dNTPs, and buffer—just add your cells and go!
- Primers are also available separately
Applications
- Yeast two-hybrid screening
- Yeast one-hybrid screening
