This kit contains reagents for making adaptor-ligated ds cDNA from poly A+ RNA and performing 5′ and 3′ Marathon RACE PCR using gene-specific primers and a mixture of thermostable DNA polymerases supplied by the user. This kit contains sufficient reagents for 5 cDNA synthesis reactions and 100 PCR reactions.
Overview
Marathon cDNA Amplification Kit (Cat. # 634913)
- Quickly obtain cDNAs without library screening
- Avoid troublesome single-stranded ligation or tailing reactions
- Perform 5′ and 3′ RACE from the same template
Applications
- RACE (Rapid Amplification of cDNA Ends)
- Identification of 3’/5′ UTRs
- Full-length gene cloning
- Gene cloning and analysis
