M13 Primers

Additional information

Brand

M13 DNA sequencing primers

M13 DNA sequencing primers enable sequencing inserts in M13mp and pUC vectors. M13 Primer M3 and M13 Primer M4 are forward DNA sequencing primers while M13 Primer RV is a reverse DNA sequencing primer.

Sequence information

  • M13 Primer M3: d(GTAAA ACGAC GGCCA GT)
  • M13 Primer M4: d(GTTTT CCCAG TCACG AC)
  • M13 Primer RV: d(CAGGA AACAG CTATG AC)

Purity

  • Confirmed by HPLC

Storage

–20°C

Activity

  •  Primer activity confirmed by Sanger dideoxy sequencing method

Product citations

Huang, Y. F., Chen, S. C., Chiang, Y. S., Chen, T. H. & Chiu, K. P. Palindromic sequence impedes sequencing-by-ligation mechanism. BMC Syst. Biol. 6 Suppl 2, S10 (2012).