Lentivirus Purification Kits

Lentivirus purification

Lentivirus purification.The Lenti-X gravity column-based method (bind, wash, elute) is extremely simple, effective, and preserves virus much better than syringe filter-based methods.

Clontech’s lentiviral purifcation kit generates high yield and high purity

Clontech’s lentiviral purification kit generates high yield and high purity. Lentivirus was generated using our high-titer lentiviral packaging system and purified. Panel A. To determine yield the lentiviral titer was measured before and after purification using either titration by qRT-PCR (RNA copies) or flow cytometry/fluorescence (IFU). The mean values from seven experiments are shown. Panel B. Equivalent amounts of purified lentivirus (1 x 105 IFU) prepared using either Lenti-X or a filter-based system (Vendor M & Vendor S) were subjected to SDS-PAGE and silver-stained. The Lenti-X sample clearly contained far fewer contaminating proteins at equivalent IFU than either sample prepared from the filter-based systems.

Collection Rack L

Collection Rack L. Once assembled, the rack holds three columns (e.g., lentiviral purification columns) and three collection tubes (50 ml conical tubes) safely and securely.