Lenti-X lentiviral vector systems with EF-1 alpha promoters allow you to achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells. The EF-1 alpha promoter, which offers a broad host range, is derived from the human EEF1A1 gene that expresses the alpha subunit of eukaryotic elongation factor 1 (Uetsuki et al. 1989; Wan Kim et al. 1990).
Options for lentiviral vectors with an EF-1 alpha promoter
The Lenti-X Expression System (EF1α Version) is a complete system that includes the pLVX-EF1α-IRES-Puro Vector, and our fourth-generation lentiviral packaging system. Alternatively, you can express your protein fused to a red (mCherry or DsRed-Monomer) or green (AcGFP1) fluorescent protein, or express your protein and a fluorescent protein separately from an internal ribosome entry site (IRES).
Overview
- High-titer lentiviral vector system
- Constitutive expression from the human EF1 alpha promoter
- Robust, long-term expression, in the broadest range of cell types
- Bright fluorescent proteins to monitor expression or localization, learn more from our fluorescent protein quick guide.
Applications
- If CMV promoters are silenced in your cell type, try EF-1 alpha
- Lentiviral transduction of stem cells
- Lentiviral transduction of hematopoietic cells
