The Lenti-X Tet-On 3G Inducible Expression System is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-G-pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system for your gene of interest in both dividing and nondividing cell types. The system provides enough reagents for 16 lentiviral packaging reactions. In the presence of doxycycline (Dox), the Tet-On 3G transactivator specifically binds and activates high-level transcription from the inducible promoter that controls expression of your gene.
Overview
- Low basal expression, high maximal expression, highly controllable
- Lenti-X Packaging Single Shots (VSV-G), included with each system, generate high titers with a very high safety profile
- TRE3G promoter provides very tight control of transcription
- Tet-One formats are also available
Applications
- Tetracycline-inducible expression in the widest range of hard-to-transfect cell types.
