The Lenti-X Integration Site Analysis Kit contains all the reagents needed for PCR-based identification of lentiviral vector integration sites in transduced cell clones. The first step is the construction of pools of adaptor-ligated genomic DNA fragments, referred to as “libraries”. These libraries can then be used for identifying genomic DNA sequences flanking the 5′ end of the integrated provirus using long-distance PCR.
Overview
- Identify sites of lentiviral integration into the host genome
- Simple determination of lentivirus integration sites using GenomeWalker technology
- Compatible with most commercially available lentivirus vectors
- Complete system including all the necessary enzymes, primers, controls, PCR kit, genomic DNA purification kit, and gel purification kit
- Retroviral version also available for detecting integration sites for MMLV and MSCV retroviruses
- Identify sites of lentiviral integration into the host genome
