Lenti-X iDimerize Reverse Dimerization System

Additional information

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The Lenti-X iDimerize Reverse Dimerization Vector Set 1 is a component of the Lenti-X iDimerize Reverse Dimer System (Cat. No. 635076). This system enables lentiviral delivery of your gene of interest into a variety of mammalian cells, and the expression of your protein of interest, tagged with a domain allowing for reversible protein dimerization/aggregation, via a small molecule “solubilizer”.

Overview

  • Brings the disruption of protein complexes under real-time, small-molecule control
  • Plasmid and lentiviral (Lenti-X) vector formats are available
  • Previously available from ARIAD as the ARGENT Regulated Secretion/Aggregation Kit
  • The D/D Solubilizer performs the same function as the AP21998 ligand, which was previously supplied by ARIAD Pharmaceuticals Inc.; however, it is a different molecule than AP21998

Applications

  • Rapid, reversible changes in the subcellular location, aggregation state, and/or biological activity of engineered proteins in vitro or in vivo
  • Rapid induction of protein secretion
  • Protein trafficking studies
  • Inducible animal models and cell lines

Rivera, V. M. et al. Regulation of protein secretion through controlled aggregation in the endoplasmic reticulum. Science 287, 826–30 (2000).

Volchuk, A. et al. Megavesicles Implicated in the Rapid Transport of Intracisternal Aggregates across the Golgi Stack. Cell 102, 335–348 (2000).