Lenti-X Concentrator

Rapid lentivirus concentration

Rapid lentivirus concentration. Add Lenti-X Concentrator reagent to clarified viral supernatant, incubate for 30 min to overnight at 4°C, and spin. That’s it.

Lentiviral concentration with high yield

Lentiviral concentration with high yield. Lentiviral supernatant from a pLVX-ZsGreen1 vector was concentrated from 3 ml down to 30 µl using the Lenti-X Concentrator Reagent. The titer before and after concentration were measured and confirmed that >90 of the lentivirus remained functional. Samples were titrated on HT1080 cells and analyzed by flow cytometry 48 hr post-transduction.

Concentrate lentivirus from any volume or from any lentiviral titer

Concentrate lentivirus from any volume or from any lentiviral titer. Lentiviral supernatant was diluted into 250 ml and then concentrated down to 2.5 ml using Lenti-X Concentrator (Panel A). 10-fold serial dilutions of a high-titer lentiviral supernatant (high, medium, and low) were concentrated from a volume of 10 ml down to 100 µl using the Lenti-X Concentrator Reagent (Panel B). Titrations were performed using HT1080 cells and flow cytometry 48 hr post-transduction.