LA Taq PCR kit
The LA PCR Kit, Version 2.1 contains all the reagents needed for amplification of long DNA templates, enabling routine amplification of products up to 20 kb in length, including GC-rich amplicons. For some DNA templates, amplification of up to 48 kb is possible. This long-range PCR kit contains TaKaRa LA Taq DNA Polymerase, buffers, MgCl2, dNTPs, molecular weight markers, and control templates, plus corresponding primers to ensure optimal PCR performance during long PCR.
Overview
- Genomic DNA template amplification up to 30 kb, with included control reactions
- Lambda DNA template amplification up to 48 kb, with included control reactions
- Longer and more accurate PCR products
- Specialized 2X GC buffer provided for templates with high GC content or extensive secondary structure
PCR products
PCR products generated with LA Taq DNA Polymerase contain a mixture of 3′-A overhangs and blunt ends, resulting in >80% cloning efficiency in T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.
