LA Taq DNA polymerase, hot-start version
This hot-start formulation consists of Takara LA Taq plus a monoclonal antibody to Taq Polymerase. It retains all of the high performance features of Takara LA Taq and provides increased reaction specificity and reduced background. The polymerase mix is optimized for amplification of long DNA templates (routinely 20 kb and up to 48 kb depending on template type) with high fidelity. Separate tubes of optimized buffer (Mg2+ plus) and dNTP mix are supplied with the polymerase.
Overview
- Robust amplification of long DNA templates (long-range PCR)—products up to 48 kb are possible
- Integrated antibody-mediated hot-start PCR—reduces non-sepcific background amplification and increases reaction specificity
- Specially optimized buffer (LA Buffer II)—less optimization is required and gives greater yield
- DNA proofreading polymerase mix—offers higher fidelity compared with conventional Taq DNA polymerase
Applications
- Long-range PCR to amplify products up to 48 kb
- Amplification of highly homologous sequences
- Pseudogene identification
- Mitochondrial DNA sequencing
PCR products
PCR products generated with Takara LA Taq contain a mixture of 3′-A overhangs and blunt ends, allowing >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.
