The iDimerize Reverse Dimerization System lets you control the subcellular localization, catalytic activity, and secretion of a protein of interest in live cells via a membrane-permeant compound. The system includes the iDimerize Reverse Dimerization Vector Set 1, which contains three mammalian expression vectors encoding fusion tags (i.e., self-assembly domains in combination with different localization sequences) that can be easily added to your protein of interest. The aggregation state of the resulting chimeric protein can be controlled by the addition of a small molecule to the cell medium. In the absence of the compound, the tagged protein expressed by the system self-assembles into complexes; when the compound is added to the cell medium, the complexes disaggregate, allowing the now soluble tagged proteins to regain their normal function. The system also includes a membrane-permeant, solubilizing compound, D/D Solubilizer, required for dissolving aggregates, and two linear selection markers for hygromycin and puromycin resistance.
Overview
- Brings the disruption of protein complexes under real-time, small-molecule control
- Plasmid and lentiviral (Lenti-X) vector formats are available
- Previously available from ARIAD as the ARGENT Regulated Secretion/Aggregation Kit
- The D/D Solubilizer performs the same function as the AP21998 ligand, which was previously supplied by ARIAD Pharmaceuticals Inc.; however, it is a different molecule than AP21998
Applications
- Rapid, reversible changes in the subcellular location, aggregation state, and/or biological activity of engineered proteins in vitro or in vivo
- Rapid induction of protein secretion
- Protein trafficking studies
- Inducible animal models and cell lines
