The iDimerize Regulated Transcription System allows tightly regulated, inducible expression of your gene of interest by the addition of a small molecule to the cell medium. The system includes the iDimerize Regulated Transcription Vector Set 2, which contains a vector that encodes two transcription factor domains (which combine to create the inducible transcription factor), a mammalian expression vector used to express your gene of interest, and a control vector. The system also includes the A/C Heterodimerizer, the membrane-permeant compound required for induction of gene expression through the heterodimerization of the two transcription factor domains.
Overview
- Tightly regulated transcription using iDimerize technology
- Dose-dependent, small molecule control of transcription
- Activation domain is physically separated from the DNA prior to addition of the dimerizer ligand, resulting in low background
- A/C Heterodimerizer is identical to the AP21967 ligand
Applications
- Inducing gene expression in a ligand-dependent manner
- Analyzing genes that promote cell death, block the cell cycle, or are otherwise toxic
- Functional genomics research
