High-purity, double-stranded cDNA for rapid cloning, sequencing, or probe generation. cDNA was synthesized using an oligo(dT) primer and purified to remove interfering RNA. The cDNA was generated from premium RNA prepared from >30 different human tissues.
Overview
- Highly purified double-stranded cDNA
- Clone genes directly by PCR, rather than library screening
- Prepared from high-quality human tissues and cell lines
- Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs
Applications
- Clone cDNAs without library screening
- Generate hybridization probes using gene-specific or degenerate primers
- Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs
Lee, C. et al. Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase. Science 239 (1988).
Parmentier, M. et al. Molecular cloning of the thyrotropin receptor. Science 246 (1989).
Schuchman, E. H., Jackson, C. E. & Desnick, R. J. Human arylsulfatase B: MOPAC cloning, nucleotide sequence of a full-length cDNA, and regions of amino acid identity with arylsulfatases A and C. Genomics 6, 149–158 (1990).
Vallins, W. J. et al. Molecular cloning of human cardiac troponin I using polymerase chain reaction. FEBS Lett. 270, 57–61 (1990).
Wilks, A. F., Kurban, R. R., Hovens, C. M. & Ralph, S. J. The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family. Gene 85, 67–74 (1989).