Human Thyroid Gland Marathon-Ready cDNA

Generation of full-length cDNA by end-to-end amplification from adaptor-ligated ds cDNA

Generation of full-length cDNA by end-to-end amplification from adaptor-ligated ds cDNA. RACE PCR was performed. The template for Lanes 1–3 was Marathon-Ready Human Placenta cDNA (Cat. # 639311); the template for Lanes 4 & 5 was Marathon-Ready Human Skeletal Muscle cDNA (Cat. # 639313). Lane 1: 5′-RACE product for TFR cDNA. Lane 2: 3′-RACE product for TFR cDNA. Lane 3: full-length TFR cDNA (5.1 kb) generated by end-to-end amplification using 5′ and 3′ TFR primers. Lane 4: nearly full-length IGFR2 cDNA (8.8 kb) amplified with 5′ IGFR2 and 3′ IGFR2 primers. Lane 5: full-length IGFR1 cDNA (5.0 kb) amplified with primers 5′-IGFR and 3′-IGFR. Lane M: 1-kb DNA size ladder.

Marathon RACE products obtained using G3PDH primers to amplify Marathon-Ready Human, Mouse, and Rat Heart cDNAs

Marathon RACE products obtained using G3PDH primers to amplify Marathon-Ready Human, Mouse, and Rat Heart cDNAs. RACE PCR was performed using the AP1 primer and the positive control G3PDH primers. The templates used were Marathon-Ready Human Heart (Cat. # 639304), Mouse Heart (Cat. # 639404), and Rat Heart (Cat. # 639416) cDNAs. Lane M: 1-kb DNA size ladder.