Cellartis Intestinal Epithelial Cells (from ChiPSC18) are small intestinal epithelial cells derived from human induced pluripotent stem (hiPS) cells. This product includes the following items: one frozen vial of Cellartis Intestinal Epithelial Cells (from ChiPSC18) and one Cellartis IEC Maturation Kit.
Overview
- Cells form tight junctions within 5 days of thawing and can be used on Days 5–8 post-thaw
- Cells express intestinal markers (e.g., villin and CDX2) at high levels
- Drug metabolizing enzyme CYP3A4 and drug transporter PEPT1 are expressed at high levels compared to Caco-2 cells
- Cells form a functional, permeable barrier that can be used to predict in vivo drug permeability and absorption
Applications
- Evaluation of drug absorption and metabolism in the small intestine
- ADME profiling in drug discovery
- Drug permeability assays
