Cellartis enhanced hiPS-HEP v2 kits provide an inexhaustible supply of mature hepatocytes derived from human induced pluripotent stem (hiPS) cells. hiPS-HEP cells have functional drug-metabolizing machinery over a 14-day assay window, making them ideal for in vitro drug discovery, drug metabolism, and toxicology-related studies. Mature, robust metabolic features support metabolic disease modeling.
The user-friendly kit format includes complete media and components for long-term assays. Compared to other hepatic cell models, these cells survive longer in culture, which enables a longer assay window in chronic toxicity experiments and for metabolic disease modeling.
Overview
- Complete kit for thawing, plating, and culturing the provided cells
- Highly homogeneous population with consistent performance between batches and over time
- Cells are functional and stable for a 14-day assay window
- Cells show long-term, stable CYP450 activities
- Cells express key hepatocyte proteins, including α1AT, Alb, CK18, HNF4α, and E-cadherin
- Cells secrete physiologically relevant levels of albumin and urea
- Cells demonstrate functional insulin and glucose regulation
- Cells show Phase I and II enzyme activities
- Cells demonstrate LDL uptake
Applications
- Toxicity testing, including chronic toxicity assays
- Drug discovery and development
- Metabolic studies
- Metabolic disease modeling
Watch the On-Demand recordings below from Dr Ralitsa Madsen and Dr Montse Morell to learn more about optimizing and speeding up your hiPSC-based disease modeling projects:
- Day 1 Short Talk 2;
“CRISPR-based hiPSC models of genetic PI3K pathway activation”
Dr Ralitsa Madsen, UCL, London, UK
- Day 2 Short Talk 1;
“Using hiPS gene editing to create a tyrosinemia disease model”
Dr Montse Morell (Takara Bio USA), Mountain View, USA
