Human Stem Cell-derived Beta Cells

Brand	Takara Bio

Cellartis hiPS beta cells have been differentiated from our ChiPSC12 and ChiPSC22 lines of control human induced pluripotent stem (hiPS) cells, dissociated into a single-cell suspension, and frozen in vials. Each kit contains beta cells, coating substrate, basal media, and a supplement.

Cellartis hiPS cell-derived beta cell kits contain beta cells generated in vitro using a standardized protocol that mimics embryonic development. These cells secrete high amounts of insulin and C-peptide, and they show substantial protein expression of insulin/C-peptide, MAFA, NKX6-1, PDX1, and UCN3. We offer two complete kits, each containing cells that originate from healthy human donors, including one with a diabetes-susceptible HLA type (HLA-A*02:01). The kits are ideal for investigating beta cell function, diabetes/disease modeling, and compound screening for insulin secretion and regulation.

Overview

Beta cells from ChiPSC12 and ChiPSC22 originate from healthy human donors
Beta cells from ChiPSC22 originate from a donor with a diabetes-susceptible HLA type, HLA-A*02:01 (Marron et al. 2002)
Beta cells from ChiPSC22 can be used alone or in combination with beta cells from ChiPSC12, which have an alternate HLA type
Cells show protein expression of insulin/C-peptide, MAFA, NKX6-1, PDX1, and UCN3
Cells demonstrate insulin and C-peptide secretion
Cells were derived with a protocol that mimics embryonic development
Cells provide reproducible and biologically relevant data

Applications

Disease modeling for diabetes
Compound screening for insulin secretion and regulation
Beta-cell functionality
GSIS (glucose-stimulated insulin secretion) analysis
Incretin response studies
Autoimmune beta-cell destruction
Pancreatitis and beta-cell function
Transdifferentiation

Donor information and HLA typification data.

Donor information and HLA typification data. The complete kits each contain cells that originate from a different donor/HLA type. ChiPSC12 was sourced from skin fibroblasts from a healthy human 24-year-old adult male volunteer (European/North African; 77 kg/177 cm). ChiPSC22 was sourced from skin fibroblasts from a healthy human 32-year-old adult male volunteer (European/North African; 74 kg/179 cm). ChiPSC22-derived beta cells originate from a human donor with a diabetes-susceptible HLA type, HLA-A*02:01. Beta cells from ChiPSC22 can be used alone or in combination with beta cells from ChiPSC12, which have an alternate HLA type.

Cellartis hiPS Beta Cells (from ChiPSC12) were exposed to incretin (100 nM GLP-1 or 100 nM exenatide) for 45 min in 5.5 mM glucose.

Cellartis hiPS Beta Cells (from ChiPSC12) were exposed to incretin (100 nM GLP-1 or 100 nM exenatide) for 45 min in 5.5 mM glucose. The content of secreted C-peptide was analyzed using the Mercodia C-peptide ELISA kit. The results are mean � SEM (n=5, GLP-1; n=4, exenatide). *p<0.05.

Human iPS cell-derived beta cells synthesize and secret insulin.

Human iPS cell-derived beta cells synthesize and secret insulin. Staining for C-peptide and insulin was performed by immunofluorescence on beta cells at Day 14 post-thawing. Colocalized insulin and C-peptide indicate intracellular insulin synthesis. Scale bar = 100 �m.

Marron, M. et al. Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice. Proc Nat Acad Sci U. S. A. 99, 13753–8 (2002).

Additional information

Overview

Applications

Donor information and HLA typification data.

Cellartis hiPS Beta Cells (from ChiPSC12) were exposed to incretin (100 nM GLP-1 or 100 nM exenatide) for 45 min in 5.5 mM glucose.

Human iPS cell-derived beta cells synthesize and secret insulin.

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