A high-quality cDNA template is necessary to obtain good results from PCR amplification. QUICK-Clone cDNA is premade double-stranded cDNA from which you can amplify sequences of interest using gene-specific primers, and is ideal for amplifying previously isolated, structurally related, or cross-species cDNAs. Synthesized from premium, high-quality poly A+ RNA from various human internal organ tissues using an oligo(dT) primer, QUICK-Clone cDNA is purified to remove residual RNA and size-selected to eliminate cDNA fragments smaller than 400 bp. QUICK-Clone cDNA allows you to amplify cDNAs of interest while avoiding traditional library construction and screening steps, and can also be used to generate hybridization probes using gene-specific or degenerate primers (Lee et al. 1988; Parmentier et al. 1989; Schuchman, Jackson, and Desnick 1990; Vallins et al. 1990; Wilks et al. 1989).
Overview
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Highly purified double-stranded cDNA
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Clone genes directly by PCR, rather than library screening
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Prepared from high-quality human tissues and cell lines
- Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs
Applications
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Clone cDNAs without library screening
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Generate hybridization probes using gene-specific or degenerate primers
- Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs
