HL-dsDNase

Heat inactivation

The HL-dsDNase can be heat inactivated by heat treatment at 5 min at 58˚C. The enzyme requires 1 mM DTT and pH ≥ 8 for complete inactivation.

Specifications

• Unit definition
  One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml of high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2.
• Specific activity
  Ca. 200 000 Kunitz Units/mg.
• Activity
  The HL-dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.
• Storage buffer
  20 mM Tris-HCl pH 7.5, 2 mM MgCl2, 10 mM NaCl, 0.01 (v/v) Triton X-100, 50 (v/v) glycerol.
• Purity
  HL-dsDNase is purified to apparent homogeneity.
• Storage
  Minimum shelf life is 2 years at -20°C. The enzyme also tolerates multiple freeze-thaw cycles.