His-tagged recombinant protein purification—His60 Ni Superflow Resin
His60 Ni Superflow Resin is a high-capacity immobilized metal affinity chromatography (IMAC) resin for the efficient purification of recombinant his-tagged proteins under native or denaturing conditions. The His60 Ni Buffer Set includes extraction, equilibration, and elution buffers optimized for the purification of his-tagged proteins on His60 Ni Superflow Resin and His60 Ni Gravity Columns.
Overview
- Up to 60 mg/ml of recombinant his-tagged protein
- Low metal ion leakage in eluted his-tagged protein
- For purification under native or denaturing conditions
- Use for batch/gravity flow and automated FPLC purification
Applications
Purified recombinant his-tagged proteins can be used for:
- Protein labeling
- Immunization to produce antibodies
- Animal studies
- Other applications
