HhaI

Brand	Takara Bio

HhaI restriction site:

G CG | C
C | GC G

Supplied buffer: M

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 37°C

Source: Haemophilus haemolyticus
Concentration: 4–12 units/µl
Substrate for unit definition: lambda DNA
Star activity: unrelated site may be cut in the presence of DMSO or high concentrations of glycerol
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning