Hepatic Cell Differentiation

Procedural overview for hepatocyte differentiation gene expression analysis by real time RT-PCR

The overall workflow for the PrimerArray Series primer sets is straightforward:

1. RNA extraction (30 min): We recommend using NucleoSpin RNA (Cat. # 740955.50) or RNAiso Plus (Cat. # 9108/9109, not available in all geographic locations). Removal of genomic DNA using Recombinant DNase I (RNase-free) (Cat. # 2270A) is recommended.
2. Reverse transcription (20 min): PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A) is recommended.
3. Dispense real-time PCR reaction solution (10 min): Prepare a master mix containing the cDNA sample and TB Green Premix Ex Taq II (Tli RNase H Plus) (Cat. # RR820A); dispense into 96-well plate.
4. Add PrimerArray primers (5 min): Use 4 µl of PrimerArray Series primers per reaction 
5. Real-time PCR (1–2 hours): PrimerArray Series primers are compatible with all commonly used real-time PCR systems 
6. Data analysis (30 minutes): Use the PrimerArray analysis tool

PrimerArray Analysis Tool for Hepatic Differentiation (Human)

The PrimerArray analysis tool facilitates data analysis and can be downloaded here. To access an instruction guide about how to use the tool, click on the Details down arrow icon in the product table, click on the Documents tab, and then expand the drop-down menu under User Manuals.

Information about RT-PCR primers for PrimerArray Hepatic Differentiation (Human)

To download primer information for this set of PrimerArray RT-PCR primers, click on the Details down arrow icon in the product table, click on the Documents tab, and then expand the drop-down menu under User Manuals.