HaeIII

Brand	Takara Bio

HaeIII restriction site:

GG | CC
CC | GG

Supplied buffer: M

Effect of DNA methylation: enzyme activity is not affected by CG methylase

Reaction temperature: 37°C

Source: Haemophilus aegyptius
Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
Substrate for unit definition: lambda DNA
Star activity: unrelated site may be cut in the presence of high concentrations of glycerol
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning