Our Glycoprotein Enrichment Resin is used for the enrichment of glycosylated proteins from serum, significantly reducing sample complexity in proteomics studies. This phenylboronic acid-based resin, which enriches glycoproteins in 90 min, is supplied in a flexible format for use with either gravity-flow columns or FPLC.
Principle
Glycoprotein Enrichment Resin is a boronic acid-based resin which provides quick, efficient, and specific enrichment of glycoproteins from complex samples such as human serum, using either simple gravity-flow columns or medium pressure methods such as FPLC. The resin consists of an m-aminophenylboronic acid ligand coupled to agarose beads. The ligand binds to cis-diol groups on sugar residues such as mannose, galactose, or glucose that are present within the saccharide moiety of glycoprotein molecules, forming a reversible five-member ring complex. This complex can be dissociated by lowering the pH, or by using an elution buffer containing either Tris or sorbitol.
Overview
- Flexible—enrichment resin can be used with gravity flow or FLPC columns
- Superior performance—enrichment resin shows increased specific binding of glycoproteins and reduced nonspecific binding
- Specific—enrichment resin enriches for low and high-abundance serum glycoproteins
- Fast—enrichment resin enriches glycoproteins in 90 min
