Rapid affinity purification of GST-tagged proteins
Affinity adsorbent-based immobilized resin based on cross-linked agarose Superflow for the purification of recombinant glutathione S-transferase (GST)-tagged biomolecules under native conditions. This resin permits one-step purification of GST-tagged recombinant proteins under flow rates up to 15 ml/ cm2·min and pressures up to 150 p.s.i. for production-scale applications.
Overview
- Easy to use—no optimization required
- Purifies more protein—high binding capacity (>10 mg of recombinant GST fusion protein per ml of resin)
- Yields highly purified protein—eluted protein is free of cellular contaminants
- Reusable—easy protocol for regenerating and storing resin
- Save money—30–40% less expensive than competing brands
Applications
- Purification of GST-tagged proteins: batch, gravity-flow, or FPLC applications
Reagents incompatible with Glutathione-Superflow Resin
These reagents are incompatible at any concentration:
- CHAPS
- DTE (dithioerythritol) and DTT (dithiothreitol)
- Guanidine hydrochloride
- SDS
- Urea
