FbaI (BclI)

Brand	Takara Bio

FbaI (BclI) restriction site:

T | GATC A
A CTAG | T

Supplied buffer: K

Effect of DNA methylation: enzyme activity is affected by dam methylase; therefore, most DNAs from E. coli are not clevable.

Reaction temperature: 37°C

Source: Flavobacterium balustinum
Concentration: 4–12 units/µl
Substrate for unit definition: N6-methyladenine free lambda DNA
Star activity: unrelated site may often be cut in the presence of high concentration of glycerol or DMSO, and at alkaline pH or low ionic strength
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning