EcoRI

Brand	Takara Bio

EcoRI restriction site:

G | AATT C
C TTAA | A

Supplied buffer: H

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 37°C

Source: Escherichia coli RY13
Concentration: 8–20 units/μl [High concentration: 30–60 units/µl]
Substrate for unit definition: lambda DNA
Enzyme activity is affected by CG methylase depending on the sequence following the recognition site
Star activity: Unrelated sites may often be cut in the presence of a high concentration of glycerol, Mn2+, or DMSO, and at low ionic strength. Enzyme activity reduces to 70 to 80 by the addition of spermine (ca. 0.2 mM), but star activity reduces to 30–50.
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning