DNA Polymerase I, Klenow Fragment
DNA Polymerase I, Klenow Fragment (Large Fragment) is a proteolytic product of E. coli DNA Polymerase I, which possesses the 5’→3′ polymerase and 3’→5′ exonuclease activities of intact DNA Polymerase I, but lacks the 5’→3′ exonuclease activity of full-length DNA Polymerase I. The lack of 5’→3′ exonuclease activity enables the DNA Polymerase I, Klenow Fragment to be used in random primer labeling and DNA sequencing experiments in addition to second-strand cDNA synthesis. The enzyme is supplied in a buffer of 50 mM potassium phosphate (pH 6.5), 1 mmDTT and 50% glyerol (2140A/B) or ethylene glycol (2140AK/BK).
Applications
- Random primer labeling
- Second-strand cDNA synthesis
- Dideoxy DNA sequencing
- 2140AK/BK are sequencing grade reagents
