DNA Ligation Kit, Mighty Mix

Brand	Takara Bio
Size	100 Rxns

Brand

Size

100 Rxns

Takara’s DNA Ligation Kit, Mighty Mix, is a one-solution premix ligation reagent that offers high efficiency ligations, particularly for blunt-ended ligation. The reaction can be performed for 5 minutes at 25°C (rapid protocol), or 30 minutes at 16°C (standard protocol) with equal results for both circular and linear DNA ligations. The 2X Ligation Mix allows small ligation reaction volumes (10 μl) when DNA is limiting. The kit includes sufficient reagent for 100 ligation reactions. Ligation products reaction can be used directly in bacterial transformation.

Overview

Enables high-efficiency ligations for cohesive, blunt-end, and TA cloning
2X ligation mix adds convenience and eliminates extra pipetting and purification steps
Ligation reactions can be quickly performed at either 5 or 30 minutes depending on DNA type
Reaction volumes are scalable

Applications

Highly efficient and fast DNA ligation
Small reaction volume ligation
TA and blunt-end cloning

Blunt-end ligation efficiency with Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors

Blunt-end ligation efficiency with Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors. Each ligation reaction contained 25 fmol of BAP-treated pUC118-Hinc II vector and 75 fmol of a 500 bp insert. Ligation efficiency was assessed by blue/white colony screening.

TA-vector ligation efficiency of Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors

TA-vector ligation efficiency of Takara-brand DNA Ligation Kit, Mighty Mix and similar kits from three competitors. Each ligation reaction contained 13 fmol of a pT7 Blue T-Vector and 39 fmol of a 2.08 kb PCR Product. Ligation efficiency was assessed by blue/white colony screening.

Recommended reaction conditions

Recommended reaction conditions.

Note: This kit retains activity through 50 freeze-thaw cycles. The Ligation Mix should be thawed on ice (5-10 minutes) and mixed well by pipetting prior to use.

Product citation

Hayashi, K., Nakazawa, M., Ishizaki, Y., Hiraoka, N. & Obayashi, A. Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol. Nucleic Acids Res. 14, 7617–31 (1986).

DNA Ligation Kit, Mighty Mix

Additional information