DNA Fragmentation Kit

Brand	Takara Bio
Size	20 Rxns

Brand

Size

20 Rxns

This kit is designed to perform random fragmentation of genomic DNAs and other long-chain dsDNAs by enzyme treatments without any special apparatus such as a sonicator, and then to blunt the obtained DNA fragment. Blunt-end fragments may be inserted into blunt-end vectors. The reaction may be stopped after fragmentation is complete if no blunting is required. This kit may also be used to perform the pretreatment in methylated DNA enrichment and high-speed DNA sequence analysis.

Applications

Genomic DNA fragmentation
dsDNA fragmentation

Components

20 preparations
Enzyme-1	20 µl
Dilution Buffer-1	1,040 µl
A Solution	20 µl
B Solution	50 µl
Stop Solution	400 µl
150 mM MgCl2	40 µl
Dilution Buffer-2	200 µl
Enzyme-2	20 µl
0.5 M EDTA	50 µl
dH2O	10 × 1 ml

Storage

–20°C. Solution A, Solution B, 150 mM MgCl2, 0.5 M EDTA and dH2O may be stored at 4°C.

Materials required but not provided

Thermal cycler (at least 1 cycler is required; 2 cyclers are preferred)

Electrophoresis loading buffer

We recommend using a loading buffer that contains a dye (e.g., Orange G) that does not overlap with dsDNA fragmentation bands ranging from 100 bp to 1,000 bp in size on the electrophoresis gel. When using BPB or xylene cyanol, please be aware that these dyes overlap with dsDNA fragments in the 100 bp to 1,000 bp size range.

Additional information

Applications

Components

Storage

Materials required but not provided

Latest news & insights.

Upcoming Webinar: Automated CRISPR Genome Editing in Human Stem Cells – Lonza

Transform Bacterial Research with Lonza’s 4D-Nucleofector® 96-Well Unit

Checkout our latest Agency Listing

The Great ESKYpade: Giving Styrofoam Eskies a Second Life

Webinar: Macherey-Nagel Microbial DNA – Purify the easy way!

Scientifix Proud Sponsors of the Adelaide Immunology Retreat Student Prize

Application

Equipment

Call 1800 007 900

Product Enquiry