The ProteoTuner DD Monoclonal Antibody recognizes the DD-N and DD-C domains, which have been optimized for use as N- and C-terminal fusion tags respectively.
Easily detect ProteoTuner shield system DD fusions with the DD Monoclonal Antibody. Cell lysates from HeLa cells transiently expressing either DD-AcGFP1 or AcGFP1-DD and HEK 293 cells stably expressing DD-AcGFP1 were analyzed by Western blot using the DD Monoclonal Antibody at a 1:500 dilution. Lane 1: HeLa cells transfected with pDD-AcGFP1 (e.g., DD-N). Lane 2: Negative control (untransfected HeLa cells). Lane 3: HeLa cells transfected with pAcGFP1-DD (e.g., DD-C). Lane 4: Negative control (untransfected HEK 293 cells). Lane 5: HEK 293 cells stably expressing DD-AcGFP1.
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