Cryonase Cold-Active Nuclease

Brand	Takara Bio

Cryonase Cold-Active Nuclease is a recombinant endonuclease originally derived from a psychrophile, Shewanella sp., and purified from E. coli. This endonuclease enzyme can complete digestion of all types of DNA and RNA (single-stranded, double-stranded, linear or circularized) at cold temperatures and even in samples placed on ice. As a result, Cryonase Cold-Active Nuclease is frequently used during DNA digestion of mixed samples, such as those that include heat-labile proteins.

Applications

Digestion of all types of DNA
Digestion of all types of RNA
Enzymatic and other heat-labile reactions

Purity

Protease activity is less than the detection limit
E. coli genomic DNA contamination is less than the detection limit

Buffer

Supplied in a buffer (20 mM NaCl, 10 mM Tris-HCl (pH 7.5), 50 glycerol, 2mM MgCl2)

Product citations

Baldermann, S. et al. Functional characterization of a carotenoid cleavage dioxygenase 1 and its relation to the carotenoid accumulation and volatile emission during the floral development of Osmanthus fragrans Lour. J. Exp. Bot. 61(11):2967–2977 (2010).

Yang, H. et al. Full Genome Sequence of Bluetongue Virus Serotype 4 from China. J. Virol. 86(23):13122–13123 (2012).

Cryonase Cold-Active Nuclease

Additional information