Monoclonal anti-c-Myc (Clone 9E10) antibody, recognizing Myc tag (410–419 of the human p62-c-Myc protein), cross-linked to immobilized protein A agarose beads. This product may be used to purify or immunoprecipitate c-Myc-tagged fusion proteins from cell lysate. The amount of cross-linked antibody on the agarose beads is >0.5 mg/ml.
Co-immunoprecipitation detection using c-Myc monoclonal antibody. Max protein tagged with a GFP variant and Myc protein tagged with a c-Myc tag were co-expressed in HEK 293 cells. Immunoprecipitations were performed with anti-GFP antibody (Lane 2) or anti-Myc antibody (Lane 4) and then detected on a Western blot using anti-Myc antibody. Lane 2 shows that Myc can be pulled down by Max. Lane 4 shows that c-Myc monoclonal antibody can effectively pull down Myc. Lanes 1 and 3 represent mock transfected lysates.
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