CloneAmp HiFi PCR Premix—high-fidelity PCR for cloning
CloneAmp HiFi PCR Premix is designed for use with the In-Fusion Cloning system due to its exceptionally accurate and efficient DNA amplification. The 2X master mix contains enzyme, optimized buffer, and dNTPs, allowing rapid setup of PCR reactions and facilitating high-throughput applications for multiple cloning samples.
Overview
- Highly accurate—exceptionally low error rate (12 mismatched bases per 542,580 total bases)
- Fast—quick priming and extension (5 sec/kb) enable fast PCR reactions
- Specific—hot-start antibody prevents nonspecific amplification
- Versatile—works with long (up to 10 kb) and GC-rich templates
- Convenient—premix allows rapid reaction setup
