ClaI

Brand	Takara Bio

ClaI restriction site:

AT | CGAT
TAGC | TA

Supplied buffer: M

Effect of DNA methylation: enzyme activity is often affected by both CG and dam methylase

Reaction temperature: 30°C

Source: Caryophanon latum L
Concentration: 10 units/µl [High Concentration: 50 units/µl]
Substrate for unit definition: lambda DNA
Genomic DNA analysis: Arthrobacter luteus genome DNA
Note: this enzyme shows equivalent activity at 37°C compared to 30°C
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning