The Cellartis DEF-CS 500 Culture System is an easy-to-use, complete culture system for defined and feeder-free iPS-cell expansion in a non-colony, 2D-monolayer format.
With this system, cells are maintained in an undifferentiated state with virtually no background differentiation, eliminating the need for cell selection. The highly reproducible nature of the system, coupled with its ability to ensure an efficient and predictable growth rate, makes the DEF-CS culture system ideal for the expansion and scale-up of a homogeneous population of iPS cells. Following reprogramming, successful expansion of your pluripotent stem cells is crucial. The DEF-CS culture system, a research-grade system, provides media, a coating reagent, and growth factors for the easy culture of iPS cells as a 2D monolayer.
Overview
- Non-colony, 2D-monolayer format enables you to culture pluripotent stem cells just like any other cell type
- Feeder-free and chemically defined
- Cells maintain pluripotency and long-term potential for self-renewal and differentiation
- Robust system provides high reproducibility, stable growth, and ensures maintenance of a normal karyotype
- Complete kit includes 500 ml of basal medium, additives, and coating compound
Applications
- Single-cell culture of human iPS cells
- Transfection and reprogramming
- High-throughput screening
- Tissue engineering (seeding cells on a scaffold)
- Gene editing
