Rapid Protein G-based antibody purification
Capturem Protein G Miniprep Columns consist of single-use, disposable columns for simple, rapid purification of antibodies that yield 50–100 μg of purified antibody per column, depending upon the sample type, species, and antibody isotype. These miniprep columns are suitable for purification of antibodies from animal sera, ascites fluid, cell culture media, and other sources. Each column can hold up to 850 µl of sample and requires a minimum elution volume of 200 µl.
Overview
- Fast and convenient: room-temperature protocol, complete in under 15 minutes, without incubation
- High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
- High quality: short residence times reduce the possibility of aggregation or loss of activity
- Flexible: purify a wide range of antibodies
Applications
- Antibody purification
- Immunoprecipitation
