Rapid, Protein A-based IP—Capturem IP & Co-IP Kit
The Capturem IP & Co-IP Kit is designed for the simple and rapid capture of protein-protein complexes from whole cell extracts. The kit contains specially designed Protein A spin columns, using our novel Capturem technology for efficient capture of antibody-bound target protein complexes. The included buffer set provides ease of use and rapid isolation of immunoprecipitated protein complexes, allowing faster downstream analysis.
Overview
- Simple, ultra-fast workflow: incubate IP antibody with sample for 10 minutes then purify at room temperature in 5 minutes
- Complete solution: includes Capturem Protein A spin columns and immunoprecipitation buffers optimized for IP and Co-IP experiments
- High concentration: extremely small elution volumes yield highly concentrated complexes
- High quality: short residence times reduce the possibility of aggregation, complex dissociation, or loss of activity
- Versatility: compatible with a wide range of IP buffers and conditions
- Single-use: novel membrane technology assembled into disposable spin columns reduces the risk of contamination and carryover
Applications
- Immunoprecipitation
- Co-immunoprecipitation
- Investigation of protein complexes
- Antigen screening for antibody discovery and engineering workflows
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