Capturem high-capacity membranes
Capturem His-Tagged Purification Maxiprep Columns are single-use disposable membrane spin columns for simple, rapid purification of his-tagged proteins in up to 25 ml of clarified lysate. The columns are suitable for use under native or denaturing conditions, and in the presence of additives such as DTT (up to 10 mM), βME (up to 30 mM), TCEP (up to 5 mM), EDTA (up to 10 mM), or glycerol.
Overview
- No-waiting workflow—5-minute, room-temperature protocol
- High purity and yield—small column bed volumes trap fewer contaminants and allow large-volume washes
- Compatible with wide range of conditions—native and denaturing conditions; with common additives (e.g., EDTA, DTT, BME, glycerol, TCEP, etc.; see compatibility table)
- Lysis buffer compatibility—xTractor Buffer; buffers from other vendors
- Different cell systems—easy purification from mammalian and bacterial cells
Applications
- Biochemical/enzymatic assays
- Pull-down or other protein interaction assays
- Confirmation screening
- Protein/ligand affinity analysis
- Crystallography or other structural analyses
- Antibody production
- Toxicology studies
- Protein transfer to live cells
