Capturem high-capacity membranes
Capturem His-tagged 24-Well Plates are 24-well plates that provide rapid, high-quality, small-scale purification of his-tagged proteins. These plates combine Ni-NTA chemistry with novel Capturem technology to provide high-capacity, membrane-based affinity purification. Typical yields for his-tagged proteins are up to 800 µg per well with a 4.5-ml sample capacity per well.
Overview
- No-waiting workflow—5-minute, room-temperature protocol
- High purity and yield—small column bed volumes trap fewer contaminants and allow large-volume washes
- Compatible with wide range of conditions—native and denaturing conditions; with common additives (e.g., EDTA, DTT, BME, glycerol, TCEP, etc.; see compatibility table)
- Lysis buffer compatibility—xTractor Buffer; buffers from other vendors
- Different cell systems—easy purification from mammalian and bacterial cells
Applications
- Biochemical/enzymatic assays
- Pull-down or other protein interaction assays
- Confirmation screening
- Protein/ligand affinity analysis
- Crystallography or other structural analyses
- Antibody production
- Toxicology studies
- Protein transfer to live cells
