BstPI (BstEII, EcoO65I)

Brand	Takara Bio

BstPI (BstEII, EcoO65I) restriction site:

G | GTNAC C
C CANTG | G

Supplied buffer: H

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 60°C

Source: Bacillus stearothermophilus
Concentration: 10 units/μl
Substrate for unit definition: lambda DNA
Star activity: unrelated site may often be cut in the presence of high concentration of glycerol and at low ionic strength
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning