BspT104I (AsuII, NspV)

Brand	Takara Bio

BspT104I (AsuII, NspV) restriction site:

TT | CG AA
AA GC | TT

Supplied buffer: L

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 37°C

Source: Bacillus species T104
Concentration: 10 units/µl
Substrate for unit definition: lambda DNA
Genome DNA analysis: Arthrobacter luteus genome DNA
Star activity: unrelated site may often be cut in the presence of high concentration of glycerol or DMSO
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning