Bpu1102I (EspI)

Brand	Takara Bio

Bpu1102 I (Esp I) restriction site:

GC | TNA GC
CG ANT | CG

Supplied buffer: Basal

Reaction temperature: 37°C

Source: Bacillus pumilus
Concentration: 10 units/ul
Substrate for unit definition: lambda DNA
Ligation-recutting test: ligation efficiency of DNA fragments with cohesive end generated by this enzyme is lower than general 3-base protruding ends. Therefore, more efficient ligation can be achieved by using the reaction conditions for blunt-end ligation
Each lot undergoes stringent quality testing, including: overdigestion, genome DNA analysis, ligation-recutting, and pKF3 Cloning