BglI

Brand	Takara Bio

BglI restriction site:

GCCN NNN | NGGC
CGGN | NNN NCCG

Supplied buffer: basal

Effect of DNA methylation: enzyme activity is affected by CG methylase

Reaction temperature: 37°C

Source: Bacillus globigii
Concentration: 10 units/µl
Substrate for unit definition: lambda DNA
Genome DNA analysis: Staphylococcus aureus genome DNA
Star activity: unrelated site may often be cut at low ionic strength
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning