BciT130 I (EcoR II, Mva I)

Brand	Takara Bio

BciT130I (EcoRII, MvaI) restriction site:

CC | (A/T)GG
GG(T/A) | CC

Supplied buffer: K

Effect of DNA methylation: enzyme activity is not affected by dcm methylase

Reaction temperature: 37°C

Source: Bacillus circulans T130
Concentration: 10 units/µl
Substrate for unit definition: lambda DNA
Inactivation treatment: Heat treatment (70°C, 15 min) is not sufficient to inactivate this enzyme completely. It is necessary to inactivate this enzyme by ethanol precipitation treatment (or phenol treatment followed by ethanol precipitation treatment)
Ligation-recutting test: After digestion with this enzyme, the ligation is inefficient under the influence of the structure of BciT130I fragment ends. For ligation, the overnight ligation using DNA Ligation Kit Ver. 2.1 is recommended.
Star activity: unrelated sites may be cut in the presence of high concentrations of glycerol, DMSO, or low ionic strength
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning