BamHI

Brand	Takara Bio

BamHI restriction site:

G | GATC C
C CTAG | G

Supplied buffer: K

Effect of DNA methylation: enzyme activity is not affected by CG methylase, dam methylase, or dcm methylase

Reaction temperature: 30°C

Source: Escherichia coli carrying the plasmid encoding BamHI gene
Concentration: 8–20 units/μl [High concentration: 30–60 units/μl]
Substrate for unit definition: lambda DNA
Star activity: unrelated site may often be cut in the presence of high concentration of glycerol, Mn2+, or DMSO, and at low ionic strength
Notice: this enzyme shows equivalent activity at 37°C and to 30°C, but the stability of the enzyme is lower in reaction mixtures at 37°C than that at 30°C
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning