BalI

Brand	Takara Bio

BalI restriction site:

TGG | CCA
ACC | GGT

Supplied buffer: basal

Effect of DNA methylation: enzyme activity is affected by dcm methylase

Reaction temperature: 37°C

Source: Brevibacterium albidum
Concentration: 1–5 units/μl
Substrate for unit definition: lambda DNA
Cleavage of pBR322: the commercially available pBR322 is substantially undigested because the cytosine residues in the recognition site (TGGCCA) are methylated by Dcm methylase
Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning