BAL 31 Nuclease

BAL 31 Nuclease

BAL 31 Nuclease acts as an exonuclease, degrading double-stranded DNA and RNA from both 5′-phosphate and 3′-hydroxyl termini. This enzyme also possesses single-stranded DNA and RNA endonuclease activity and is capable of cleaving at DNA/RNA nicks and gaps. It requires Ca2+ as an essential cofactor for its exonuclease and endonuclease activities.

BAL 31 Nuclease is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 5 mM CaCl2, 5 mM MgCl2, 1 mM EDTA and 50% glycerol.

Applications

• Progressive deletion of DNA and RNA fragments